Studies on phospholipids. 5. The separation of dinitrophenylated and methylated phospholipids by countercurrent distribution*

Hplc Separation of Phospholipids

The principles involved in the selection of solvents and the use of counter ions, together with the choice of column, for the separation of phospholipids by highperformance liquid chromatography (HPLC) are described.

Separation of phospholipids and individual molecular species of phospholipids by high-performance liquid chromatography.

Isocratic high-performance liquid chromatography methods are described for separating the major classes of phospholipids and for isolating the individual molecular species of phospholipids. Fractionation of a total lipid extract of rat liver on a silica column resulted in quantitative recoveries of all major phospholipids with preservation of their fatty acid composition. Rat liver phosphatidyl...

Physical Studies of Phospholipids

On heating pure, fully saturated 2,3-diacyl-DL-phosphatidyl-ethanolamines and 2,3-diacylphosphatidyl-cholines (lecithins) in water to the transition temperature at which large endothermic heat changes occur, they are observed, by light microscopy, to form myelin figures. This result is discussed in terms of the large difference in the transition temperature for "melting" of the hydrocarbon chai...

Countercurrent-distribution studies on histones.

1. The possibilities of fractionating histones and histone fractions by means of countercurrent distribution between two phases formed by water and butan-2-ol, in the presence of various concentrations of trichloroacetic acid, have been examined. 2. Although the principal histone fractions differ considerably in their partition ratios, a satisfactory resolution of the principal histone fraction...

Phospholipids. 3. On the chromatographic separation of glycerophospholipids.